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KMID : 0385219970070030048
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Korean Journal of Gerontology
1997 Volume.7 No. 3 p.48 ~ p.54
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Effects of Rhizoma Gastrodiae and Ramulus et Uncus Uncariae on cultured vestibular nucleus neurons damaged by oxygen radicals in vestibular compensation
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Park Seung-Taeck
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Abstract
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To elucidate the mechanism of vestibular compensation on cultured rat vestibular nucleus(VN) neurons damaged buy oxidant-induced neurotoxicity in unilateral labyrinthectomy(UL), we examined the neurotoxicity induced by oxygen radicals by MTT assay when cultured VN neurons were grown in the medium containing various concentrations of xanthine oxidase(XO) and hypoxanthine (HX) for 3 hours. In addition, neuroprotrctive effects of herb extracts such Rhizoma Gastrodiae and Ramulus et Uncus Uncariae on oxidant-induced neurotoxicity in cultured VN neurons were evaluated by MTT assay after cultured VN Neurons were preincubated with various concentrations of herb extract, Rhizoma Gastrodiae or Ramulus et Uncus Uncariae for 2 hours before 20 mU/㎖ XO and 0.1 HX for 3 hours. Oxygen radicals decreased remarkably cell viability in dose and time-dependent manner in these cultures and also herb extract, Rhizoma Gastrodiae increased cell viability of VN neurons damaged by oxygen radicals. While Ramulus et Uncus Uncariae did not show any protective effect on oxidant-induced neurotoxicity. From the above results, it is suggested that oxygen radicals are toxic in cultured VN neurons derived from rat and Rhizoma Gastrodiae is effective in blocking the nuerotoxicity induced by oxidative stress in these cultures.
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KEYWORD
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Unilateral labyrinthectomy, Oxidative stress, Vestibular nucleus neuron, Antioxidant
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